DNA Sample Integrity of Breathable Bags
Tests for migration of DNA from samples of bodily fluids through the breathable membrane were performed under controlled conditions, for 24 hours, 1 week and 2 weeks.
In Test Set A, reproducing the worst conditions of use, the breathable bag was enclosed in a larger polyethylene bag to minimise external contamination and a weight placed on top of the sample to ensure maximum contact area between the wet sample and the surface.
In Test set B, representing ‘standard’ drying conditions, the breathable membrane surface was exposed directly to the surrounding air. Weights were placed on the corners of the bag to ensure sample contact; with minimum reduction of the surface area exposed.
Swabs from the section of breathable membrane above the sample were taken at intervals of 24 hours, 7 days and 2 weeks. The results were compared with the DNA profiles of the original samples from the start and end of the tests. External contamination was detected, but there was insignificant transfer to the interior surface.
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Results
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Test A
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Test B
|
|
External Contamination
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DNA Migration
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External Contamination
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DNA Migration
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| 24 Hours |
|
|
|
|
| Blood |
No
|
No
|
Yes
|
No
|
| Saliva |
No
|
No
|
Yes
|
No
|
| Semen |
No
|
No
|
Yes
|
No
|
| 7 Days |
|
|
|
|
| Blood |
No
|
Yes
|
Yes
|
No
|
| Saliva |
No
|
Yes
|
Yes
|
No
|
| Semen |
No
|
Yes
|
Yes
|
No
|
| 14 Days |
|
|
|
|
| Blood |
No
|
Yes
|
Yes
|
No
|
| Saliva |
No
|
Yes
|
Yes
|
No
|
| Semen |
No
|
Yes
|
Yes
|
No
|
The results indicate that there is a ‘latency’ period of approximately 24 hours before DNA migration from the sample reaches the surface of the membrane. It is recommended that this should be the maximum time between collection of evidence and transfer to controlled drying conditions.
They also confirm that the breathable surface of the bag must be exposed to surrounding air, to ensure there is no DNA migration across it during the drying period and that there is no significant transfer of external contamination to the inner surface.
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